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Labor sexual division causes inequality between genders, as well as situations of violence directed at women in the workplace. In the pharmaceutical area, the female majority, and the insertion in multiple scenarios, which involve contact with the public and different professional partnerships, increase exposure to violence. Therefore, the objective of this study was to analyze the characteristics of situations of violence experienced by women pharmacists in the workplace. For this, a qualitative approach was carried out based on the content analysis of an online survey, answered by women pharmacists (n=381) registered with the Regional Council of Pharmacy of Minas Gerais state, Brazil. The NVivo® software was used. Four categories emerged: “Living with disrespect, threats and vulnerability”, “Sexism and structural machismo that silence, diminishes and stuns”, “Discrimination against women as an obstacle to equity”, “Sexual harassment and objectification of women”. Several expressions of violence emerged in the workplace and coming from multiple aggressors. Women pharmacists acknowledged being professionally harmed and little recognized for their technical capacity. Lack of protection of pharmaceutical integrity in the work environment was reported. It is hoped that these results will shed light on gender inequality in the pharmaceutical workplace, with emphasis on violence, broadening the discussion and providing evolution in this mostly female profession.